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pspgrna-dync1h1-mut  (Addgene inc)


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    Structured Review

    Addgene inc pspgrna-dync1h1-mut
    Pspgrna Dync1h1 Mut, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pspgrna+dync1h1+mut/pspgrna+dync1h1+mut/pm36550020-68-7-22
    Average 90 stars, based on 1 article reviews
    pspgrna-dync1h1-mut - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Control:

    Article Title: Non-Synonymous, Synonymous, and Non-Coding Nucleotide Variants Contribute to Recurrently Altered Biological Processes During Retinoblastoma Progression
    Article Snippet: The identified gRNA coding sequence (gagga C gtcggtgatgaagg; targeted C underlined) was inserted into pSPgRNA (Addgene plasmid # 47108). .. CHLA-VC-RB31 cells were co-transfected with the resulting pSPgRNA- DYNC1H1-mut or a control pSPgRNA expressing non-targeting gRNA and dCas9-cytosine base editor vector pCAG-CBE4max-SpG-P2A-EGFP (RTW4552) (Addgene plasmid # 139998) using the Neon transfection system with 5 μg of each plasmid, 2 × 10 cells in 100 μl tips, and transfected with 2 pulses of 20 ms at 1100 V. After 24 hours culture, transfected GFP+ cells were sorted to yield bulk GFP+ cultures and single cell clones. ..

    Expressing:

    Article Title: Non-Synonymous, Synonymous, and Non-Coding Nucleotide Variants Contribute to Recurrently Altered Biological Processes During Retinoblastoma Progression
    Article Snippet: The identified gRNA coding sequence (gagga C gtcggtgatgaagg; targeted C underlined) was inserted into pSPgRNA (Addgene plasmid # 47108). .. CHLA-VC-RB31 cells were co-transfected with the resulting pSPgRNA- DYNC1H1-mut or a control pSPgRNA expressing non-targeting gRNA and dCas9-cytosine base editor vector pCAG-CBE4max-SpG-P2A-EGFP (RTW4552) (Addgene plasmid # 139998) using the Neon transfection system with 5 μg of each plasmid, 2 × 10 cells in 100 μl tips, and transfected with 2 pulses of 20 ms at 1100 V. After 24 hours culture, transfected GFP+ cells were sorted to yield bulk GFP+ cultures and single cell clones. ..

    Plasmid Preparation:

    Article Title: Non-Synonymous, Synonymous, and Non-Coding Nucleotide Variants Contribute to Recurrently Altered Biological Processes During Retinoblastoma Progression
    Article Snippet: The identified gRNA coding sequence (gagga C gtcggtgatgaagg; targeted C underlined) was inserted into pSPgRNA (Addgene plasmid # 47108). .. CHLA-VC-RB31 cells were co-transfected with the resulting pSPgRNA- DYNC1H1-mut or a control pSPgRNA expressing non-targeting gRNA and dCas9-cytosine base editor vector pCAG-CBE4max-SpG-P2A-EGFP (RTW4552) (Addgene plasmid # 139998) using the Neon transfection system with 5 μg of each plasmid, 2 × 10 cells in 100 μl tips, and transfected with 2 pulses of 20 ms at 1100 V. After 24 hours culture, transfected GFP+ cells were sorted to yield bulk GFP+ cultures and single cell clones. ..

    Transfection:

    Article Title: Non-Synonymous, Synonymous, and Non-Coding Nucleotide Variants Contribute to Recurrently Altered Biological Processes During Retinoblastoma Progression
    Article Snippet: The identified gRNA coding sequence (gagga C gtcggtgatgaagg; targeted C underlined) was inserted into pSPgRNA (Addgene plasmid # 47108). .. CHLA-VC-RB31 cells were co-transfected with the resulting pSPgRNA- DYNC1H1-mut or a control pSPgRNA expressing non-targeting gRNA and dCas9-cytosine base editor vector pCAG-CBE4max-SpG-P2A-EGFP (RTW4552) (Addgene plasmid # 139998) using the Neon transfection system with 5 μg of each plasmid, 2 × 10 cells in 100 μl tips, and transfected with 2 pulses of 20 ms at 1100 V. After 24 hours culture, transfected GFP+ cells were sorted to yield bulk GFP+ cultures and single cell clones. ..

    Single Cell:

    Article Title: Non-Synonymous, Synonymous, and Non-Coding Nucleotide Variants Contribute to Recurrently Altered Biological Processes During Retinoblastoma Progression
    Article Snippet: The identified gRNA coding sequence (gagga C gtcggtgatgaagg; targeted C underlined) was inserted into pSPgRNA (Addgene plasmid # 47108). .. CHLA-VC-RB31 cells were co-transfected with the resulting pSPgRNA- DYNC1H1-mut or a control pSPgRNA expressing non-targeting gRNA and dCas9-cytosine base editor vector pCAG-CBE4max-SpG-P2A-EGFP (RTW4552) (Addgene plasmid # 139998) using the Neon transfection system with 5 μg of each plasmid, 2 × 10 cells in 100 μl tips, and transfected with 2 pulses of 20 ms at 1100 V. After 24 hours culture, transfected GFP+ cells were sorted to yield bulk GFP+ cultures and single cell clones. ..

    Clone Assay:

    Article Title: Non-Synonymous, Synonymous, and Non-Coding Nucleotide Variants Contribute to Recurrently Altered Biological Processes During Retinoblastoma Progression
    Article Snippet: The identified gRNA coding sequence (gagga C gtcggtgatgaagg; targeted C underlined) was inserted into pSPgRNA (Addgene plasmid # 47108). .. CHLA-VC-RB31 cells were co-transfected with the resulting pSPgRNA- DYNC1H1-mut or a control pSPgRNA expressing non-targeting gRNA and dCas9-cytosine base editor vector pCAG-CBE4max-SpG-P2A-EGFP (RTW4552) (Addgene plasmid # 139998) using the Neon transfection system with 5 μg of each plasmid, 2 × 10 cells in 100 μl tips, and transfected with 2 pulses of 20 ms at 1100 V. After 24 hours culture, transfected GFP+ cells were sorted to yield bulk GFP+ cultures and single cell clones. ..



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